Efficient manual Fmoc solid-phase synthesis of the N-terminal segment of surfactant protein B (SP-B(1-25)).

نویسندگان

  • Suria Ramli
  • Ian R Gentle
  • Benjamin P Ross
چکیده

The N-terminal 25 residue segment of human surfactant protein B (SP-B(1-25)) was synthesised in 26% yield by manual Fmoc solid-phase peptide synthesis (Fmoc SPPS) using low-loading Fmoc-Gly-Wang resin. Substantial oxidation of Met(21) occurred during the synthesis, and the addition of Bu(4)NBr to a TFA/water/EDT/TIS cleavage cocktail enabled facile reduction of Met(O)(21)-SP-B(1-25) to SP-B(1-25). The methods described herein are generally applicable to the Fmoc SPPS of difficult sequences containing methionine.

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عنوان ژورنال:
  • Protein and peptide letters

دوره 16 7  شماره 

صفحات  -

تاریخ انتشار 2009